mouse anti futsch 22c10  (Developmental Studies Hybridoma Bank)

Journal: PLOS One

Article Title: Sequoia affects Drosophila central nervous system development by regulating axonal extension and guidance

doi: 10.1371/journal.pone.0333573

Figure Lengend Snippet: The Futsch protein is expressed in all sensory neurons. The 22C10 antibody was used to track the development and axonal migration of the Futsch‑positive neurons. (A) In normal embryos, sensory neurons migrate from the PNS toward the CNS in three main groups: dorsal (D), lateral (L) and ventral (V). (B) The phenotype of sensory neurons in GA168 homozygotes at developmental stage 16. The migration of sensory axons toward the CNS is affected, seen as a loss of connection between the three groups of sensory neurons (black arrowhead). The lateral neurons have reduced cilia extensions (arrowhead). (C) In C3101, the number of sensory neurons is reduced, and particularly, there is a marked reduction in the number of lateral group neurons, which might be stalled dorsally, or these groups are missing in C3101 (black arrowhead). (D) In H156, the axons from the dorsal cluster fail to extend properly and the fascicles are thinner in some segments (arrowhead). (E) In C022 there are strong axonal guidance defects in dorsal cluster axons, sometimes crossing the segmental boundaries (arrowhead). (F) In Z1241, severe defects are detected with a general disorganization of the whole PNS. These phenotypes were imaged at a magnification power of 20X. Scale bar, 10 μm. (G, H) Detail of the lateral clusters displaying the PNS axonal extension phenotypes. (I) Quantification of axonal guidance errors from the dorsal cluster (n = 20 embryos).

Article Snippet: We used BP102 antibody from DSHB and antibodies that recognize Futsch (22C10 - DSHB), Fasciclin II (1D4 - DSHB), Eve (3C10 - DSHB), FasIII (7G10 – DSHB), Sequoia [ ], GFP (Molecular Probes and Roche), ßGal (Cappel and Promega), and Cy2, Cy3 and Cy5-conjugated secondary antibodies (Jackson ImmunoResearch).

Techniques: Migration

Journal: bioRxiv

Article Title: Sex specific systemic effects of sev-Gal4 driven activated Ras expression mediated through hnRNPs in Drosophila

doi: 10.64898/2026.02.27.708457

Figure Lengend Snippet: a-d” . Confocal optical sections showing distribution of 22C10 antibody stained Futsch in proximal posterior regions of 120hr larval eye discs in sev > eGFP female (a-a”) and male (b-b”) , sev > Ras V12 female (c-c”) and male (d-d”) larvae; eGFP and DAPI fluorescence are shown in green and blue, respectively. e . Dot-plots of Futsch fluorescence intensity (Y-axis) in female and male eye discs in different genotypes (X-axis)

Article Snippet: Following primary antibodies were used: rabbit monoclonal anti-Ras (27H5, Cell signaling, 1:100), rabbit polyclonal anti-TBPH (10782-2-AP, Proteintech, 1:100), mouse monoclonal anti Caz (3F4,1:100, provided by Moushami Mallik, Germany [ ], mouse monoclonal anti-Sxl (M114-s, Developmental Studies Hybridoma Bank, 1:20), mouse anti-Futsch/22c10-s (AB_528403, Research Resource Identifier, 1:20), and mouse monoclonal anti-FasII (1D4-s, Developmental Studies Hybridoma Bank, 1:20).

Techniques: Staining, Fluorescence